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pET41-FeoB-OL2/4sub
(Plasmid #216746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET41-a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    feoB
  • Species
    P. aeruginosa (Bacteria)
  • Mutation
    Outer loop 4 (OL4) with the sequence of [TLDGKPVQGDIGHSALASVSQRLTPLLAPLGVQPDNWQATVGLVTGAMAKEVVVGTLNTLYTAEQIQGEAFDYEGYDLPGQLRDALVETWDGLVDTFSLSVLANPVEASMADGDMETGSMGTMASKFGSPI] is substituted with OL2 [LEDSGYMARAAFVMDRLMQ].
  • Entrez Gene
    PA4358 (a.k.a. PA4358)
  • Tag / Fusion Protein
    • 8x His (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Henrietta Venter: Sansom Institute for Health Research, University of South Australia, Adelaide, Australia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IPTG inducible

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET41-FeoB-OL2/4sub was a gift from Gordon Laurie (Addgene plasmid # 216746 ; http://n2t.net/addgene:216746 ; RRID:Addgene_216746)
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