pET41-FeoB-OL4del
(Plasmid
#216744)
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PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET41-a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namefeoB
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SpeciesP. aeruginosa (Bacteria)
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MutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQGDIGHSALASVSQRLTPLLAPLGVQPDNWQATVGLVTGAMAKEVVVGTLNTLYTAEQIQGEAFDYEGYDLPGQLRDALVETWDGLVDTFSLSVLANPVEASMADGDMETGSMGTMASKFGSPI] is deleted.
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Entrez GenePA4358 (a.k.a. PA4358)
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Tag
/ Fusion Protein
- 8x His (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Henrietta Venter: Sansom Institute for Health Research, University of South Australia, Adelaide, Australia
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IPTG inducible
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET41-FeoB-OL4del was a gift from Gordon Laurie (Addgene plasmid # 216744 ; http://n2t.net/addgene:216744 ; RRID:Addgene_216744)