pET41-FeoB-OL1del
(Plasmid #216741)

• Purpose: Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.
• Depositing Lab: Gordon Laurie
• Publication: feoB and potH plasmids (unpublished)

Backbone

• Vector backbone: pET41-a(+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: feoB
• Species: P. aeruginosa (Bacteria)
• Mutation: Outer loop 1 (OL1) with the sequence of [INIGGALQP] is deleted.
• Entrez Gene: PA4358 (a.k.a. PA4358)
• Tag / Fusion Protein:
  • 8x His (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Henrietta Venter: Sansom Institute for Health Research, University of South Australia, Adelaide, Australia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IPTG inducible