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Addgene

pX551-miniCMV-SpCas9D10A nickase
(Plasmid #216735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-D10A nickase
  • Species
    S pyogenes
  • Mutation
    D10A
  • Promoter T7 and mini-CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alex Hewitt (Addgene #107031 pX551-miniCMV-SpCas9)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX551-miniCMV-SpCas9D10A nickase was a gift from Vincent Dion (Addgene plasmid # 216735 ; http://n2t.net/addgene:216735 ; RRID:Addgene_216735)

  • For your References section:

    Cas9 nickase-mediated contraction of CAG/CTG repeats at multiple disease loci. Alvaro Murillo, Melanie Alpaugh, Meghan Larin, Emma L. Randall, Laura Heraty, Ruban Rex Durairaj, Alys N. Aston, Alysha S. Taylor, Alex Mas Monteys, Nina Stöberl, Aeverie E. R. Heuchan, Pascale Aeschlimann, Soumyasree Bhattacharyya, Nicholas D. Allen, Jack Puymirat, Beverly L. Davidson, Francesca Cicchetti, Mariah Lelos, Vincent Dion. bioRxiv 10.1101/2024.02.19.580669
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