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Addgene

pAAV-U6-sgCTG-CMV-GFP
(Plasmid #216734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgCTG
  • gRNA/shRNA sequence
    CTGCTGCTGCTGCTGCTG
  • Species
    Synthetic
  • Promoter U6 and CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-sgCTG-CMV-GFP was a gift from Vincent Dion (Addgene plasmid # 216734 ; http://n2t.net/addgene:216734 ; RRID:Addgene_216734)

  • For your References section:

    Cas9 nickase-mediated contraction of CAG/CTG repeats at multiple disease loci. Alvaro Murillo, Melanie Alpaugh, Meghan Larin, Emma L. Randall, Laura Heraty, Ruban Rex Durairaj, Alys N. Aston, Alysha S. Taylor, Alex Mas Monteys, Nina Stöberl, Aeverie E. R. Heuchan, Pascale Aeschlimann, Soumyasree Bhattacharyya, Nicholas D. Allen, Jack Puymirat, Beverly L. Davidson, Francesca Cicchetti, Mariah Lelos, Vincent Dion. bioRxiv 10.1101/2024.02.19.580669
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