AID-C12*-dCas9-BE4max
(Plasmid
#216731)
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PurposeExpresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAID-C12*-BE4max
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAID-C12*-dCas9-BE4max
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SpeciesH. sapiens (human)
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Entrez GeneAICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AID-C12*-dCas9-BE4max was a gift from Rahul Kohli (Addgene plasmid # 216731 ; http://n2t.net/addgene:216731 ; RRID:Addgene_216731) -
For your References section:
Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors. Berrios KN, Barka A, Gill J, Serrano JC, Bailer PF, Parker JB, Evitt NH, Gajula KS, Shi J, Kohli RM. Nucleic Acids Res. 2024 Jan 23:gkae024. doi: 10.1093/nar/gkae024. 10.1093/nar/gkae024 PubMed 38261989