pFP5380 LAMP1-SBP- mCherry-FIREtag
(Plasmid #216725)

• Purpose: Expresses LAMP1-mCherry-FIREtag in mamallian cells
• Depositing Lab: Franck Perez
• Publication: Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s41592-023-01988-8.

Backbone

• Vector backbone: pmCherry-C1
• Total vector size (bp): 6002
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP-1
• Species: H. sapiens (human)
• GenBank ID: NM_005561.4 NM_005561.4
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Streptavidin Binding Protein (SBP) (C terminal on insert)
  • mCherry (without SbfI) (C terminal on insert)
  • FIREtag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCMV_F

Resource Information

• Supplemental Documents:
  • 216725.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry