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Addgene

FP5369 KIF17MD-flag- FIREmate
(Plasmid #216724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Total vector size (bp) 5998
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF17
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2028
  • Mutation
    1-547
  • GenBank ID
    57576
  • Entrez Gene
    KIF17 (a.k.a. KIF17B, KIF3X, KLP-2, OSM-3)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (C terminal on insert)
    • FIREmate (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pEGFP-FP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FP5369 KIF17MD-flag- FIREmate was a gift from Franck Perez (Addgene plasmid # 216724 ; http://n2t.net/addgene:216724 ; RRID:Addgene_216724)

  • For your References section:

    A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity. Bottone S, Joliot O, Cakil ZV, El Hajji L, Rakotoarison LM, Boncompain G, Perez F, Gautier A. Nat Methods. 2023 Aug 28. doi: 10.1038/s41592-023-01988-8. 10.1038/s41592-023-01988-8 PubMed 37640938
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