T7-LacO-Halo-PILATeS-RGD (200nM)
(Plasmid
#216716)
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PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJ414
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Backbone manufacturerATUM
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag-PILATeS-RGD
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SpeciesSynthetic
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Insert Size (bp)1300
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Tags
/ Fusion Proteins
- HaloTag (N terminal on backbone)
- 6xHis (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This PILATeS module contains BiT tag with 200 nM affinity for LgBiT
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7-LacO-Halo-PILATeS-RGD (200nM) was a gift from Alex Dunn (Addgene plasmid # 216716 ; http://n2t.net/addgene:216716 ; RRID:Addgene_216716) -
For your References section:
Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems. Zhong BL, Elliot JM, Wang P, Li H, Hall RN, Wang B, Prakash M, Dunn AR. ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7. 10.1021/acssensors.3c02664 PubMed 38973210
Map uploaded by the depositor.
