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Addgene

pDEST14-Cys-SpyCatcher (Min)
(Plasmid #216713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST14
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cys-SpyCatcher (Min)
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    312
  • Mutation
    See Li...Howarth, J. Mol. Biol., 2014
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Truncated SpyCatcher sequence was reported in Li...Howarth, J. Mol. Biol., 2014

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST14-Cys-SpyCatcher (Min) was a gift from Alex Dunn (Addgene plasmid # 216713 ; http://n2t.net/addgene:216713 ; RRID:Addgene_216713)

  • For your References section:

    Facile detection of mechanical forces across proteins in cells with STReTCh. Zhong BL, Vachharajani VT, Dunn AR. Cell Rep Methods. 2022 Sep 2;2(9):100278. doi: 10.1016/j.crmeth.2022.100278. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100278 PubMed 36160040
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