T7-LacO-Halo-RGD-STReTCh (Force Inert)
(Plasmid
#216710)
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PurposeExpresses HaloTag-RGD-STReTCh Force Inert Control in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJ414
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Backbone manufacturerATUM
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHaloTag-RGD-STReTCh
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SpeciesSynthetic
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Insert Size (bp)1300
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Tags
/ Fusion Proteins
- HaloTag (N terminal on backbone)
- 6xHis (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7-LacO-Halo-RGD-STReTCh (Force Inert) was a gift from Alex Dunn (Addgene plasmid # 216710 ; http://n2t.net/addgene:216710 ; RRID:Addgene_216710) -
For your References section:
Facile detection of mechanical forces across proteins in cells with STReTCh. Zhong BL, Vachharajani VT, Dunn AR. Cell Rep Methods. 2022 Sep 2;2(9):100278. doi: 10.1016/j.crmeth.2022.100278. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100278 PubMed 36160040