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Addgene

pmCherryC1-Pacer
(Plasmid #216698)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherryC1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pacer
  • Species
    H. sapiens (human)
  • Entrez Gene
    RUBCNL (a.k.a. C13orf18, KIAA0226L, PACER)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherryC1-Pacer was a gift from James Hurley (Addgene plasmid # 216698 ; http://n2t.net/addgene:216698 ; RRID:Addgene_216698)
  • For your References section:

    A RAB7A phosphoswitch coordinates Rubicon Homology protein regulation of Parkin-dependent mitophagy. Tudorica DA, Basak B, Puerta Cordova AS, Khuu G, Rose K, Lazarou M, Holzbaur ELF, Hurley JH. J Cell Biol. 2024 Jul 1;223(7):e202309015. doi: 10.1083/jcb.202309015. Epub 2024 May 10. 10.1083/jcb.202309015 PubMed 38728007