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pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag
(Plasmid #216695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES_neo3
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6212
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FIREmate-KDEL
  • Insert Size (bp)
    528
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7_forward
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ManII-SBP-mApple-FIREtag
  • Alt name
    MAN2A1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1404
  • Mutation
    contains only amino acids 1 to 116 of ManII
  • Entrez Gene
    Man2a1 (a.k.a. Mana-2, Mana2, Map-2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin Binding Protein (SBP) (C terminal on insert)
    • mApple (C terminal on insert)
    • FIREtag (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer IRES-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag was a gift from Franck Perez (Addgene plasmid # 216695 ; http://n2t.net/addgene:216695 ; RRID:Addgene_216695)

  • For your References section:

    A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity. Bottone S, Joliot O, Cakil ZV, El Hajji L, Rakotoarison LM, Boncompain G, Perez F, Gautier A. Nat Methods. 2023 Aug 28. doi: 10.1038/s41592-023-01988-8. 10.1038/s41592-023-01988-8 PubMed 37640938
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