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Addgene

HA-E2F-1 wt-pRcCMV
(Plasmid #21667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRcCMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5541
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1333
  • Mutation
    wild type-
  • GenBank ID
    NM_005225
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-E2F-1 wt-pRcCMV was a gift from William Kaelin (Addgene plasmid # 21667 ; http://n2t.net/addgene:21667 ; RRID:Addgene_21667)

  • For your References section:

    Negative regulation of the growth-promoting transcription factor E2F-1 by a stably bound cyclin A-dependent protein kinase. Krek W, Ewen ME, Shirodkar S, Arany Z, Kaelin WG, Livingston DM. Cell. 1994 Jul 15. 78(1):161-72. 10.1016/0092-8674(94)90582-7 PubMed 8033208
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