pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro
(Plasmid
#216666)
-
PurposeExpresses tTa-inducible MyoD and miRFP703, with puromycin resistance
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 216666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR
- Backbone size w/o insert (bp) 9591
- Total vector size (bp) 12183
-
Vector typeLentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameMyoD
-
Alt nameMyoD1
-
Alt namemyogenic differentiation 1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1062
-
GenBank IDNM_010866.2
-
Entrez GeneMyod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)
Gene/Insert 2
-
Gene/Insert namemiRFP703
-
Insert Size (bp)972
Gene/Insert 3
-
Gene/Insert namePuroR
-
Insert Size (bp)1360
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro was a gift from Leonardo Morsut (Addgene plasmid # 216666 ; http://n2t.net/addgene:216666 ; RRID:Addgene_216666)