pNTI823
(Plasmid
#216632)
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PurposeCal-ID variant with T195/G196 split site, mammalian expression and lentivirus production
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
- Backbone size w/o insert (bp) 9225
- Total vector size (bp) 10788
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCal-ID (split site variant)
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SpeciesSynthetic
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Insert Size (bp)1605
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNTI823 was a gift from Nicholas Ingolia (Addgene plasmid # 216632 ; http://n2t.net/addgene:216632 ; RRID:Addgene_216632) -
For your References section:
Molecular recording of calcium signals via calcium-dependent proximity labeling. Kim J.W., Yong A.J.H., Aisenberg E.E. et al.. Nat Chem Biol (2024) 10.1038/s41589-024-01603-7