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Addgene

pNTI821
(Plasmid #216630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSBtet-Hyg
  • Backbone size w/o insert (bp) 6011
  • Total vector size (bp) 7616
  • Vector type
    Mammalian Expression ; Sleeping Beauty Transposon System
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cal-ID
  • Species
    Synthetic
  • Insert Size (bp)
    1605
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNTI821 was a gift from Nicholas Ingolia (Addgene plasmid # 216630 ; http://n2t.net/addgene:216630 ; RRID:Addgene_216630)

  • For your References section:

    Molecular recording of calcium signals via calcium-dependent proximity labeling. Kim J.W., Yong A.J.H., Aisenberg E.E. et al.. Nat Chem Biol (2024) 10.1038/s41589-024-01603-7
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