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Addgene

MO70 Orai1 N223A
(Plasmid #21663)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MO70
  • Backbone size w/o insert (bp) 7480
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Orai1
  • Species
    H. sapiens (human)
  • Mutation
    N223A
  • GenBank ID
    BC015369
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MO70 Orai1 N223A was a gift from Anjana Rao (Addgene plasmid # 21663 ; http://n2t.net/addgene:21663 ; RRID:Addgene_21663)

  • For your References section:

    Biochemical and functional characterization of Orai proteins. Gwack Y, Srikanth S, Feske S, Cruz-Guilloty F, Oh-hora M, Neems DS, Hogan PG, Rao A. J Biol Chem. 2007 Jun 1. 282(22):16232-43. 10.1074/jbc.M609630200 PubMed 17293345
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