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pOP01-UTC-7.0
(Plasmid #216625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AmpR-ColE1
  • Backbone manufacturer
    John Dueber lab
  • Total vector size (bp) 3110
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7p-5'g10-NanoLuc-3'TMV
  • Alt name
    nluc
  • Species
    Synthetic; Oplophorus gracilirostris
  • Insert Size (bp)
    519
  • Promoter T7

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer GCTCACATGTTCTTTCCTGC
  • 3′ sequencing primer GGTAACTGTCAGACCAAGTTTAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOP01-UTC-7.0 was a gift from Henrike Niederholtmeyer (Addgene plasmid # 216625 ; http://n2t.net/addgene:216625 ; RRID:Addgene_216625)

  • For your References section:

    Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform. Bohm CV, Inckemann R, Burgis M, Baumann J, Brinkmann CK, Lipinska KE, Gilles S, Freudigmann J, Seiler VN, Clark LG, Jewett MC, Voll LM, Niederholtmeyer H. ACS Synth Biol. 2024 Aug 16;13(8):2412-2424. doi: 10.1021/acssynbio.4c00117. Epub 2024 Jul 19. 10.1021/acssynbio.4c00117 PubMed 39028299
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