MO70 Orai1 E190Q
(Plasmid #21662)

• Depositing Lab: Anjana Rao
• Publication: Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-43.

Backbone

• Vector backbone: MO70
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Orai1
• Species: H. sapiens (human)
• Mutation: E190Q
• GenBank ID: BC015369
• Entrez Gene: ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: IRES-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See author's map for image of empty MO70 vector (MO70 contains IRES-GFP after the multiple cloning site).