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Addgene

BRAF - P453A in pENTR
(Plasmid #216610)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216610 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR8-GW-TOPO
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Gateway Entry Plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRAF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2300
  • Mutation
    P453A
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert contains a Kozak sequence that encodes a linker for cloning into N-terminal tagging pDEST vectors

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BRAF - P453A in pENTR was a gift from Douglas Allan & Christopher Loewen (Addgene plasmid # 216610 ; http://n2t.net/addgene:216610 ; RRID:Addgene_216610)
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