BRAF - C251Y in pENTR
(Plasmid
#216609)
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Purposegateway cloning donor vector containing BRAF ASD mutation
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216609 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR8-GW-TOPO
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 2800
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Vector typeGateway Entry Plasmid
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRAF
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SpeciesH. sapiens (human)
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Insert Size (bp)2300
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MutationC251Y
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Entrez GeneBRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
insert contains a Kozak sequence that encodes a linker for cloning into N-terminal tagging pDEST vectors
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BRAF - C251Y in pENTR was a gift from Douglas Allan & Christopher Loewen (Addgene plasmid # 216609 ; http://n2t.net/addgene:216609 ; RRID:Addgene_216609)