pMSCV PIG IMP-1-short
(Plasmid #21659)

• Depositing Lab: David Bartel
• Publication: Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Backbone

• Vector backbone: pMSCV PIG
• Backbone size w/o insert (bp): 7600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IGF-II mRNA-binding protein 1
• Alt name: IMP-1
• Alt name: CM18
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Entrez Gene: IGF2BP1 (a.k.a. CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1)
• Tags / Fusion Proteins:
  • IRES (C terminal on backbone)
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Xho I (unknown if destroyed)
• 5′ sequencing primer: pBABE 5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Short means always shortest 3’UTR due to alternative polyadenylation.

Constructs for Soft-agar assay