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Addgene

pMSCV Cyclin D2-short
(Plasmid #21655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCVpuro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6295
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cyclin D2
  • Alt name
    CM14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    CCND2 (a.k.a. KIAK0002, MPPH3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Hpa I (not destroyed)
  • 5′ sequencing primer pBABE 5
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Short means always shortest 3’UTR due to alternative polyadenylation.

Constructs for cell cycle

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV Cyclin D2-short was a gift from David Bartel (Addgene plasmid # 21655 ; http://n2t.net/addgene:21655 ; RRID:Addgene_21655)

  • For your References section:

    Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394
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