pWZL-hygro-FAKWT
(Plasmid #216540)

• Purpose: This retroviral plasmid expresses the human wild type PTK2
• Depositing Lab: Georgia Konstantinidou
• Publication: Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/s44321-024-00138-7.

Backbone

• Vector backbone: pWZL-Hygro
• Backbone manufacturer: Jay Morgenstern
• Backbone size w/o insert (bp): 5642
• Total vector size (bp): 5642
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTK2
• Alt name: FAK
• Species: H. sapiens (human)
• Insert Size (bp): 3243
• Mutation: wild type sequence
• GenBank ID: NP_005598.3
• Entrez Gene: PTK2 (a.k.a. FADK, FADK 1, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK)
• Tag / Fusion Protein:
  • No

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: pBABE 5'

Resource Information

• Supplemental Documents:
  • pWZL-Hygro-FAK WT.gbk
• A portion of this plasmid was derived from a plasmid made by: Ordered the mutant sequences from genscript and sub-cloned it to pWZL Hygro (Plasmid #18750).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry