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Addgene

pU6-sgRNA_MET_uORF2
(Plasmid #216538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pegRNA MET 5'UTR uORF2 mutation
  • gRNA/shRNA sequence
    GAGCGCTTTGTGAGCAGATG

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-sgRNA_MET_uORF2 was a gift from Paolo Comoglio (Addgene plasmid # 216538 ; http://n2t.net/addgene:216538 ; RRID:Addgene_216538)

  • For your References section:

    The integrated stress response drives MET oncogene overexpression in cancers. Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM. EMBO J. 7 Jan 2025. doi: 10.1038/s44318-024-00338-4. 10.1038/s44318-024-00338-4
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