pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40
(Plasmid #216533)

• Purpose: An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch
• Depositing Lab: Yuki Kambe
• Publication: Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475. doi: 10.1101/2023.08.23.554475. Preprint.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5116
• Total vector size (bp): 6461
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: axon-jGCaMP8m
• Insert Size (bp): 1345
• Promoter: hSynapsin1
• Tag / Fusion Protein:
  • GAP43 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: GCAACGTGCTGGTTATTGTG (CAG-F)
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA (WPRE-R)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry