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Addgene

T7-LacO-Halo-STReTCh-RGD
(Plasmid #216532)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216532 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ414
  • Backbone manufacturer
    ATUM
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag-STReTCh-RGD
  • Species
    Synthetic
  • Insert Size (bp)
    1300
  • Tags / Fusion Proteins
    • HaloTag (N terminal on backbone)
    • 6xHis (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-LacO-Halo-STReTCh-RGD was a gift from Alex Dunn (Addgene plasmid # 216532 ; http://n2t.net/addgene:216532 ; RRID:Addgene_216532)

  • For your References section:

    Facile detection of mechanical forces across proteins in cells with STReTCh. Zhong BL, Vachharajani VT, Dunn AR. Cell Rep Methods. 2022 Sep 2;2(9):100278. doi: 10.1016/j.crmeth.2022.100278. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100278 PubMed 36160040
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