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pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
(Plasmid #216531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAVS1-NDi-CRISPRi (Gen1)
  • Backbone manufacturer
    Bruce Conklin (Addgene plasmid # 73497)
  • Total vector size (bp) 14519
  • Modifications to backbone
    KRAB-dCas9-mCherry cassette removed (cut w/ Afill and Agel)
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABI-dCas9-mCherry
  • Insert Size (bp)
    5952
  • Promoter TRE3G
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Afill (not destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ABI-dCas9-mCherry insert comes from Stanley Qi (Addgene plasmid # 121513: pHR-PGK-ABI-dCas9-P2A-mcherry)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry) was a gift from Makiko Iwafuchi (Addgene plasmid # 216531 ; http://n2t.net/addgene:216531 ; RRID:Addgene_216531)

  • For your References section:

    Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate. Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M. Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 10.1016/j.molcel.2023.12.007. 10.1016/j.molcel.2023.12.007 PubMed 38211589
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