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Addgene

pYES2/NTA/Leu-LegA7
(Plasmid #216518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2/NTA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6038
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB Carbenicillin Plates
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Leg A7
  • Species
    Legionella pneumophila Philadelphia 1
  • Insert Size (bp)
    1544
  • Mutation
    LegA7 WT
  • GenBank ID
    AAU26500.1
  • Tag / Fusion Protein
    • HIS-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2/NTA/Leu-LegA7 was a gift from Ralph Isberg (Addgene plasmid # 216518 ; http://n2t.net/addgene:216518 ; RRID:Addgene_216518)
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