hnRNPAB X2-RBD
(Plasmid
#216516)
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PurposeExpresses MBP-tagged RNA binding domain of hnRNPAB X2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTHMT
- Backbone size w/o insert (bp) 6457
- Total vector size (bp) 6943
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehnRNPAB X2-RBD
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SpeciesX. laevis (frog)
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Insert Size (bp)486
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Mutationdeleted amino acids 2-64 and 226-304
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Tag
/ Fusion Protein
- 6xHis tags-MBP (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.11.10.566489v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hnRNPAB X2-RBD was a gift from Nicolas Fawzi (Addgene plasmid # 216516 ; http://n2t.net/addgene:216516 ; RRID:Addgene_216516) -
For your References section:
Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in Xenopus oocytes. O'Connell LC, Johnson V, Otis JP, Hutton AK, Murthy AC, Liang MC, Wang SH, Fawzi NL, Mowry KL. Sci Rep. 2024 Nov 13;14(1):27890. doi: 10.1038/s41598-024-79409-9. 10.1038/s41598-024-79409-9 PubMed 39537752