pIS1 DICER1 long-mut-let-7
(Plasmid #21651)

• Depositing Lab: David Bartel
• Publication: Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Backbone

• Vector backbone: pIS1
• Backbone manufacturer: Available from Addgene (#12179)
• Backbone size w/o insert (bp): 4085
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dicer 1
• Alt name: CM10
• Species: H. sapiens (human)
• Insert Size (bp): 4200
• Mutation: Mutant let-7 microRNA binding sites
• Entrez Gene: DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
• Tag / Fusion Protein:
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Long means that all functional alternative polyadenylation signals have been deleted.

Mut means that all microRNA binding sites for an indicated microRNA have been mutated.