pcDNA3 Mito-Ras-LOCKR-S
(Plasmid
#216501)
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PurposeFRET-based reporter for Ras activity, mitochondria localized
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerGenscript
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMito-Ras-LOCKR-S
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SpeciesH. sapiens (human)
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Insert Size (bp)3300
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 Mito-Ras-LOCKR-S was a gift from David Baker (Addgene plasmid # 216501 ; http://n2t.net/addgene:216501 ; RRID:Addgene_216501) -
For your References section:
Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution. Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D. Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w. 10.1038/s41587-023-02107-w PubMed 38273065
Map uploaded by the depositor.
