pcDNA3 Golgi-Ras-LOCKR-S
(Plasmid
#216499)
-
PurposeFRET-based reporter for Ras activity, Golgi localized
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3
-
Backbone manufacturerGenscript
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameGolgi-Ras-LOCKR-S
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3309
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byGenscript
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3 Golgi-Ras-LOCKR-S was a gift from David Baker (Addgene plasmid # 216499 ; http://n2t.net/addgene:216499 ; RRID:Addgene_216499) -
For your References section:
Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution. Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D. Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w. 10.1038/s41587-023-02107-w PubMed 38273065
Map uploaded by the depositor.
