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Addgene

pcDNA3 Ras-LOCKR-S R89L (negative control)
(Plasmid #216497)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216497 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Genscript
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ras-LOCKR-S R89L negative control
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3213

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Ras-LOCKR-S R89L (negative control) was a gift from David Baker (Addgene plasmid # 216497 ; http://n2t.net/addgene:216497 ; RRID:Addgene_216497)

  • For your References section:

    Computationally designed sensors detect endogenous Ras activity and signaling effectors at subcellular resolution. Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D. Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w. 10.1038/s41587-023-02107-w PubMed 38273065
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