pET3C-ARL16(CO)-His6
(Plasmid
#216485)
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PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET3C
- Backbone size w/o insert (bp) 4638
- Total vector size (bp) 5196
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameADP-ribosylation factor-like 16
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Alt nameARL16
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SpeciesH. sapiens (human)
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Insert Size (bp)558
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GenBank IDNM_001040025.3 339231
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Entrez GeneARL16
- Promoter T7
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Tag
/ Fusion Protein
- includes GSGSGSHHHHHH (His6) tag at C-terminus (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET3C-ARL16(CO)-His6 was a gift from Richard Kahn (Addgene plasmid # 216485 ; http://n2t.net/addgene:216485 ; RRID:Addgene_216485)
