pcDNA3.1-ARL16-His6
(Plasmid #216484)

• Purpose: Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag
• Depositing Lab: Richard Kahn
• Publication: pcDNA3.1-HsARL16-His6 (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 5986
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ADP-ribosylation factor like 16
• Alt name: ARL16
• Species: H. sapiens (human)
• Insert Size (bp): 558
• GenBank ID: NM_001040025.3 339231
• Entrez Gene: ARL16
• Promoter: CMV
• Tag / Fusion Protein:
  • includes GSGSGSHHHHHH (His6) tag at C-terminus (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • 2_HsARL16-Cterm His-pcDNA3.1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry