G555
(Plasmid #216483)

• Purpose: Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.
• Depositing Lab: Christophe Danelon
• Publication: MOSAIC: a highly efficient, one-step recombineering approach to plasmid editing and diversification (unpublished)

Backbone

• Vector backbone: pUC57-oriL191-oriR194
• Backbone size w/o insert (bp): 3162
• Total vector size (bp): 9401
• Vector type: Cell-free expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: plsB, plsC, cdsA, pssA
• Species: E. coli
• Insert Size (bp): 6239
• Promoter: T7 for each gene

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • G555.gb
• A portion of this plasmid was derived from a plasmid made by: contains 2 small DNA sequences (oriL191 and oriR194) that were obtained from GenScript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.22.586135 for bioRxiv preprint.