pPOM041_P15678_SingleGeneBackbone
(Plasmid
#216466)
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Purpose(Empty Backbone) Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216466 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK089
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Vector typeYeast Expression, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOM041_P15678_SingleGeneBackbone was a gift from Tomáš Pluskal (Addgene plasmid # 216466 ; http://n2t.net/addgene:216466 ; RRID:Addgene_216466) -
For your References section:
POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T. ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529. 10.1021/acssynbio.3c00529 PubMed 37991801