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pPOM030_P4_tSynth27
(Plasmid #216455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Terminator synth27
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    79
  • Mutation
    Removal of any BsaI // BsmBI restriction site

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPOM030_P4_tSynth27 was a gift from Tomáš Pluskal (Addgene plasmid # 216455 ; http://n2t.net/addgene:216455 ; RRID:Addgene_216455)

  • For your References section:

    POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T. ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529. 10.1021/acssynbio.3c00529 PubMed 37991801
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