pPOM028_P4_tSynth3
(Plasmid
#216453)
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PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTerminator synth3
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)82
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MutationRemoval of any BsaI // BsmBI restriction site
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPOM028_P4_tSynth3 was a gift from Tomáš Pluskal (Addgene plasmid # 216453 ; http://n2t.net/addgene:216453 ; RRID:Addgene_216453) -
For your References section:
POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology. Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T. ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529. 10.1021/acssynbio.3c00529 PubMed 37991801