pIS1 Cyclin D2 short UTR
(Plasmid #21644)

• Depositing Lab: David Bartel
• Publication: Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Backbone

• Vector backbone: pIS1
• Backbone manufacturer: Available from Addgene (#12179)
• Backbone size w/o insert (bp): 4085
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyclin D2 short UTR
• Alt name: CM5
• Alt name: Cyclin D2
• Species: H. sapiens (human)
• Insert Size (bp): 200
• Entrez Gene: CCND2 (a.k.a. KIAK0002, MPPH3)
• Tag / Fusion Protein:
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EBV Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Short means always shortest 3’UTR due to alternative polyadenylation.