Skip to main content
Addgene

pIS1 IMP-1 rc
(Plasmid #21643)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIS1
  • Backbone manufacturer
    Available from Addgene (#12179)
  • Backbone size w/o insert (bp) 4085
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IGF-II mRNA-binding protein 1
  • Alt name
    IMP-1 reverse complement
  • Alt name
    CM4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6700
  • Entrez Gene
    IGF2BP1 (a.k.a. CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1)
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIS1 IMP-1 rc was a gift from David Bartel (Addgene plasmid # 21643 ; http://n2t.net/addgene:21643 ; RRID:Addgene_21643)

  • For your References section:

    Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394
Related items:
Commonly requested with: