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pPbU6_hdhfr/yfcu_Cas9
(Plasmid #216423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYCm
  • Backbone manufacturer
    Zhang (2017) 10.1016/j.molbiopara.2016.12.010
  • Backbone size (bp) 10000
  • Modifications to backbone
    The Plasmodium yoelii U6 promoter that guides gRNA expression was replaced with the Plasmodium berghei homolog
  • Vector type
    Bacterial Expression, CRISPR ; Expression in Plasmodium berghei
  • Selectable markers
    Pyrimethamine (positive selection) and 5-fluorocytosine (negative selection)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPbU6_hdhfr/yfcu_Cas9 was a gift from Ellen Bushell (Addgene plasmid # 216423 ; http://n2t.net/addgene:216423 ; RRID:Addgene_216423)

  • For your References section:

    A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei. Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC. bioRxiv 2024.04.20.590404 10.1101/2024.04.20.590404
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