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pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
(Plasmid #216421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYCs
  • Backbone manufacturer
    Qian (2018) 10.1016/j.molbiopara.2018.04.003
  • Backbone size (bp) 6000
  • Modifications to backbone
    The Plasmodium yoelii U6 promoter was changed for the Plasmodium berghei promoter to express the gRNA. A 3xmyc tag followed by the hsp70 3'UTR was added after the synthetic insert cloning site.
  • Vector type
    Bacterial Expression, CRISPR ; Expression in Plasmodium berghei
  • Selectable markers
    Pyrimethamine (positive) and 5-fluorocytosine (negative)
  • Tag / Fusion Protein
    • 3xmyc (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu was a gift from Ellen Bushell (Addgene plasmid # 216421 ; http://n2t.net/addgene:216421 ; RRID:Addgene_216421)

  • For your References section:

    A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei. Jonsdottir TK, Paoletta MS, Ishizaki T, Hernandez S, Ivanova M, Curbelo AH, Saiki PA, Selinger M, Das D, Henriksson J, Bushell ESC. bioRxiv 2024.04.20.590404 10.1101/2024.04.20.590404
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