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Efg1-ΔDBD-Y115S/Y132S/Y139S
(Plasmid #216408)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ411
  • Backbone size w/o insert (bp) 4005
  • Total vector size (bp) 5142
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Efg1-ΔDBD-Y115S/Y132S/Y139S
  • Species
    Candida albicans (Yeast)
  • Insert Size (bp)
    1137
  • Mutation
    changed Tyrosine 115, 132 and 139 to Serine, and deleted amino acids 182-356
  • Tag / Fusion Protein
    • 6xHis-tags (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.09.566450 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Efg1-ΔDBD-Y115S/Y132S/Y139S was a gift from Nicolas Fawzi (Addgene plasmid # 216408 ; http://n2t.net/addgene:216408 ; RRID:Addgene_216408)

  • For your References section:

    Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation. Wang SH, Zheng T, Fawzi NL. bioRxiv [Preprint]. 2023 Nov 9:2023.11.09.566450. doi: 10.1101/2023.11.09.566450. 10.1101/2023.11.09.566450 PubMed 37986834
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