PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11
(Plasmid
#216394)
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Purpose(Empty Backbone) dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCR4-TOPO
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Backbone manufacturerInvitrogen
- Backbone size (bp) 13909
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Vector typeMammalian Expression, Mouse Targeting, CRISPR
- Promoter Human beta-globin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11 was a gift from Evgeny Kvon (Addgene plasmid # 216394 ; http://n2t.net/addgene:216394 ; RRID:Addgene_216394) -
For your References section:
Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live Mice. Hollingsworth EW, Liu TA, Jacinto SH, Chen CX, Alcantara JA, Kvon EZ. bioRxiv 2023.12.10.570890 10.1101/2023.12.10.570890
Map uploaded by the depositor.
