modRNAc1-Cas9-2A-puro
(Plasmid
#216390)
-
Purposethis vector can be used to make modRNA expressing Cas9 and puro resistance gene
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 216390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneModRNAc1
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCas9-2A-puro
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.09.07.556774v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
modRNAc1-Cas9-2A-puro was a gift from Xiaojun Lian (Addgene plasmid # 216390 ; http://n2t.net/addgene:216390 ; RRID:Addgene_216390) -
For your References section:
MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells. Haideri T, Lin J, Bao X, Lian XL. Stem Cell Reports, 2024 10.1016/j.stemcr.2024.03.005