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6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet
(Plasmid #216386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFduet-1
  • Backbone manufacturer
    Novagen/EMD Biosciences
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dishevelled-2
  • Alt name
    Dvl2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Dvl2
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-MBP-TEV (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet was a gift from William Weis (Addgene plasmid # 216386 ; http://n2t.net/addgene:216386 ; RRID:Addgene_216386)

  • For your References section:

    PI(4,5)P(2)-stimulated positive feedback drives the recruitment of Dishevelled to Frizzled in Wnt-beta-catenin signaling. Mahoney JP, Bruguera ES, Vasishtha M, Killingsworth LB, Kyaw S, Weis WI. Sci Signal. 2022 Aug 23;15(748):eabo2820. doi: 10.1126/scisignal.abo2820. Epub 2022 Aug 23. 10.1126/scisignal.abo2820 PubMed 35998232
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