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MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
(Plasmid #216385)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGP67a
  • Backbone manufacturer
    BD Biosciences
  • Vector type
    Insect Expression ; Baculovirus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Norrin
  • Alt name
    NDP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    C93A/C95A/C131A
  • Entrez Gene
    NDP (a.k.a. EVR2, FEVR, ND)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • GP64 signal sequence-MBP-3C (N terminal on insert)
    • Rho1D4 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.03.578714 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a was a gift from William Weis (Addgene plasmid # 216385 ; http://n2t.net/addgene:216385 ; RRID:Addgene_216385)

  • For your References section:

    The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling. Bruguera ES, Mahoney JP, Weis WI. bioRxiv 2024.02.03.578714 10.1101/2024.02.03.578714
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